Development of monoclonal antibody-based immunoassay for rapid detection of sheep and deer/elk in rendered meats

Title: Development of monoclonal antibody-based immunoassay for rapid detection of sheep and deer/elk in rendered meats

Principle Investigator: Peggy Hsieh

Year: 2002

Objective: (1) Preparing and Characterizing Antigens: Characterize the species-specific region on sheep and deer/elk troponin I from sheep and deer/elk muscle, respectively and identify other major species marker proteins from sheep and deer/elk muscle

(2) Developing Antibodies:
a) Develop ovine-specific monoclonal antibodies (Mabs) raised against the marker protein(s)
b) Develop deer/elk-specific monoclonal antibodies (Mabs) raised against the marker protein(s)

(3) Characterizing Antibodies:
a) Select a pair of suitable ovine-specific Mabs for the construction of a sandwich enzyme-linked immunosorbent assay (ELISA)
b) Select a pair of suitable deer/elk-specific Mabs for the construction of a sandwich enzyme-linked immunosorbent assay (ELISA)